Many biological processes produce only one quantitative outcome per year, resulting from temperatures and precipitation during hundreds of days leading up to the event. Traditional regression approaches incur problems in such a setting, because independent variables are highly autocorrelated and their number often greatly exceeds the number of observations. Partial Least Squares Regression (PLS), a statistical analysis tool developed to handle these situations and widely used in hyperspectral remote sensing, was tested for its usefulness for explaining the climate responses of biological processes, using walnut phenology in California as an example.